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Exact(4)
The column efficiency determined from the analyte peak >20,000, the tailing factor <2.0, and relative standard deviation (RSD) for impurity areas in six replicate injection of system suitability solution was <5.0%.
Protein identifications were accepted if they could be established at ≥95% probability and contained at least two identified unique peptides across replicate injection for each condition.
Replicate injection of a concentration series of an inhibitor results in negative peaks, the height of which is related to the extent of protease inhibition.
Glycogen phosphorylase was added to the digests to a final concentration of 20 fmol/μl as an internal standard for normalization prior to each replicate injection.
Similar(56)
For calculation of the intra-day precision, this standard solution was injected five times, whereas the inter-day precision was based on three replicate injections on five consecutive days.
Six replicate injections were given for MQC sample.
The reproducibility of the method was determined by five replicate injections performed within 3 days.
The RSD of 5 replicate injections of standard RA solutions (20.9 mg/L) was also investigated.
The method was evaluated for linearity, limit of quantitation, solution stability, and precision of replicate injections.
The RSD% of six replicate injections for each analyte were reasonably good.
The accuracy (% difference from nominal) and precision (% relative standard deviation) of replicate injections were within 17.0%.
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