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SNO was measured by duplicate injection into the appropriate solution at either 30°C (I3−) or 50°C (Cu(I /Cys).
We used duplicate injection (DI) of the same sample extract, duplicate analysis of an aliquot of the same sample (DA), and analysis of DS to assess precision of the instrumental method, the analysis method, and the overall collection and analysis methods, respectively, for selected As forms.
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Duplicate injections of each sample were made.
The samples were diluted to approximately 60 mg L−1 P prior to analysis and analyzed in duplicate injections.
Subsequently internal standard correction according to the strategy described previously [32], normalization to mouse liver weight, and averaging the duplicate injections was performed.
For kinetic analysis, duplicate injections of analytes (Png1p and Png1p-ΔH1) in various concentrations (80 1000 nM) were applied to the immobilized chip at a flow rate of 5 µl/min under the buffer condition of 10 mM HEPES, pH 7.4.
For kinetic analysis, duplicate injections of analytes [recMoPrP 89 230) and recMoPrP 23 230)] in various concentrations (0.44 4.4 µM) were run under the buffer condition of HBS-N (10 mM HEPES, 150 mM NaCl), pH 7.4 (Biacore AB).
Corrected peptide intensities were averaged across duplicate injections at each time point in the 20 nM bortezomib time course.
The mean AUC for duplicate injections was used to determine the SNO concentration, with reference to a GSNO standard curve.
All signals, obtained for differing concentrations of both protamine and heparin, exhibit good reproducibility for duplicate injections and reversibility.
Both solutions of Zr-labeled immunoconjugate were diluted to 300 μL with 0.1 M ammonium acetate for duplicate injections.
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