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The resulting PP1 activity correlates with the level of released active PP1.
Active Polymer Index (API) was defined as the ratio between band areas at 978 918 cm−1 and 850 785 cm−1 of FTIR tests to characterize the released active polymer from vulcanized crumb rubber.
However, hypotonic lysis of DCs after internalization of inactive Renilla luciferase released active enzyme, suggesting that DCs were similarly capable of cytosolic refolding of internalized proteins.
Furthermore, films incubated at 60 °C for 2 and 5 months still released active nanofibers and closer examination showed that the released peptide could still form the interwoven and entangled nanofibers.
Vancomycin-loaded cylinders released active antibiotic for the first 22 hours at levels sufficient to inhibit bacterial growth in the incubation vessel as these vancomycin-containing vessels did not go on to reach turbidity if allowed to incubate further.
Chaudhuri et al. [ 49] conjugated fullerenol C60(OH 16 24 and doxorubicin (Ful-DOX) which was found to be relatively stable in phosphate buffer saline but temporally released active drug when incubated with tumor cell lysate.
Similar(52)
Surprisingly, digestion in vitro did not release active compounds from bread; the activities of enriched bread were significantly lower than those determined for buffer extracts.
Both Cry and Cyt toxins are synthesized as protoxins that are activated by insect gut proteases releasing active toxin fragments (Pardo-López et al. 2013).
It can cleave a number of extracellular substrates, including proteoglycans, to release active glycosaminoglycans [ 50], which in turn modulate its activity.
Other receptors, in particular G-protein-coupled receptors including gonadotrophin-releasing hormone receptor, caused increased activity of the ADAM family of metalloproteinases, which release active ligands from the cell surface by proteolysis [ 11].
Prior to electrochemical measurements, the nanocatalysts were activated in 1 M KOH to remove any dissolved oxygen and release active sites.
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