The 8E7 monoclonal antibody recognising active, unphosphorylated β-catenin was a kind gift of Prof Hans Clevers (Hubrecht Institute, UMC, Utrecht, Netherlands).
Cells were fixed in ethanol and after washing in 1 × PBS the amount of active caspase-3 was assessed by staining digitonin-permeabilised cells with a FITC-conjugated antibody recognising active caspase-3 (BD Pharmingen, Franklin Lakes, NJ, USA).
En face staining using antibodies that recognise active, cleaved caspase-3 (apoptosis) or using Sudan IV (lipid deposition) revealed that genetic deletion of JNK1 reduced EC apoptosis and lesion formation in hypercholesterolemic mice.
It has previously been recognised that active, hands-on training opportunities that demonstrate the experience, skills and roles of various providers working together are most effective [ 14].
Individuals need no longer be passive human 'subjects' but can be engaged over time and recognised as active, interested and valued research participants.
Rather, as Webber has pointed out, 'nurses need to be recognised as active players whose involvements are among those structuring, reinforcing, or resisting their current realities' (39, p. 9).
Antibodies that recognise active, cleaved caspase-3 were used (9661S; Cell Signalling Technology) [10].
One principle for classifying models of faith is according to the extent to which they recognise an active component in faith itself, and the way they identify that active component and its relation to faith's other components.
64 A constructivist grounded theory approach directs attention to the complexity of the world we live in by accounting for multiple perspectives on and interpretations of social phenomena (eg, recovery from critical illness) and recognising participants' active engagement in 'making sense' of their experiences.
Milonov was recognised for his "active lawmaking efforts and many years of honest work".
