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Thermally expandable microcapsules (TEMs) with wrinkled shells are prepared by one-step suspension polymerization, allowing for encapsulation and controlled release of cargos.
However, in initial studies, release of cargos from these polymeric materials was entirely diffusion controlled.
This further demonstrates that these protein-loaded nanogels have the potential to be internalized by cells, and subsequent control over the intracellular release of cargos using an external light can be easily envisioned.
For example, light-switchable α-cyclodextrin (α-CD) and azobenzene interaction has been employed to control the viscosity of hydrogels, the release of cargos from carriers, and the assembly of macroscopic/microscopic objects.
To test our hypothesis that conjugating multiple cationic dendrimers on a nanoworm scaffold would enhance the proton sponge effect, leading to better cytosolic release of cargos, we evaluated the efficiency of dendriworm endosomal escape compared to free dendrimers.
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The preferential release of cargo in acidic conditions provided enhanced transfection efficiency compared to non-degradable counterparts.
In a world with the Internet, cryptography and the blockchain, shouldn't there be a better way to enable the secure release of cargo internationally?
Submicron-scaled P-Aggs were constructed by mixing SA and cargo proteins labeled with a biotinylated caging reagent (BCR) and were utilized as a facile and versatile platform for the light-induced release of cargo proteins (Fig. 4).
In the previous experiments, we found that the combination chemoimmunotherapy of anti-CD40 plus bufalin by liposomal carriers could enhance anticancer therapeutic efficacy while reducing systemic toxicity, due to the confined biodistribution and prolonged release of cargo [12].
Because AuNPs packed densely within the nanohydrogel, their surface plasmon resonance shifted to the near-infrared (NIR) range, thereby allowing the NIR laser-mediated spatiotemporal photothermal release of cargo from temperature-sensitive liposomes [59].
This powerful concept has been successfully applied to the fabrication of drug delivery schemes where the tissue of interest is targeted via release of cargo triggered by the biocatalytic action of an enzyme.
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