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DOI: http://dx.doi.org/10.7554/eLife.04052.018 To further characterize the permeability barrier within the NPC, we determined whether impβ and RanGTP affect the free diffusion of cargos through the pore under both normal and reduced levels of Nup153.
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These FG-domains constitute the key components of the selective gate-barrier that limits the diffusion of cargoes through the NPC (see following section).
The diffusion of molecular cargo through the hydrogel matrix and the release characteristics from these hydrogels were investigated.
The complete protein import cycle can be summarized as follows (Fig. 3): (i) Importin-β proteins bind their import substrates in the cytoplasm, then, the affinity of transport receptors for the FG-repeat meshwork allows facilitated diffusion of importin-cargo complexes across the NPC.
(F ) Diffusion of a soluble cargo across a stack through transient intercisternal tubules.
Such an active motor-based delivery strategy offers dramatic improvement in the efficiency compared to the common passive diffusion of orally administrated cargoes.
Carrying out a photo-bleaching experiment to compare the kinetics of intra Golgi diffusion of small and large cargos may help to control for this in physiological conditions.
However, in initial studies, release of cargos from these polymeric materials was entirely diffusion controlled.
NPCs allow free diffusion of small molecules and can mediate active transport of cargo up to ∼39 nm in diameter (Panté and Kann, 2002).
He had sensed the diffusion of interest.
The early diffusion of Buddhism in China remains unclear.
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