Structures of interest were reconstructed and quantified from the serial EM images, with the 3D reconstruction software, Reconstruct (http://synapses.clm.utexas.edu/tools/index.stm) (Fiala, 2005).
Datasets were reconstructed using cone-beam reconstruction software (Skyscan NRecom) based on the Feldkamp algorithm [ 24] and segmented into binary images using adaptive local thresholding.
The selected genes were input into a Bayesian network reconstruction software package, RIMBANet-Reconstructing Integrative Molecular Bayesian Network [ 65], based on a previously described algorithm [ 65- 68].
Acquired projection images were reconstructed using system-supplied General Electric Datos reconstruction software.
Radionuclide images were reconstructed using HiSPECT (Scivis GmbH) iterative reconstruction software and fused with CT images using proprietary InVivoScope (Bioscan, Washington, DC, USA) software.
Radionuclide images were reconstructed using HiSPECT (Scivis, Göttingen, Germany) iterative reconstruction software and fused with CT images using proprietary InVivoScope (Bioscan) software.
3D SPECT images are reconstructed from the SPECT list-mode data with MILabs reconstruction software version 2.38.
U-SPECT-II reconstruction software version 2.38 (MiLabs, Utrecht, The Netherlands) was used to reconstruct images [27].
3D images were reconstructed with a voxel average size of 10 μm, using the manufacturer reconstruction software (NRecon, Skyscan).
Reconstruction was undertaken with system-supplied Datos reconstruction software.
Reconstruction software Flash 3D and Hybrid Recon were used.
