Below the gel diffusion rate, cells died throughout the tissue depth during extensive matrix consolidation without cracks.
To determine the growth rate, cells were seeded on six-well culture plates and maintained in their respective culture medium.
Their model proposes characteristic time constants that describe the rate cells can self-adjust the magnitude and direction of the force dipole in response to cyclic stretch.
To investigate whether down-regulation of cell-cycle genes could affect growth and proliferation rate, cells were counted daily for 5 days.
Cells exhibiting these pattern types have been found to occur anatomically intermixed in the cortex [4], with cue- and response-coupled cells appearing to be more common than fixed delay rate cells [5].
For the evaluation of cell proliferation rate, cells were seeded on 24-well plates at a density of 2×104 cells/well in medium, followed by the methyl thiazol tetrazolium assay (MTT assay; Sigma-Aldrich Co).
