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Many lower peaks exist in other velocity cells, owing to i2 t) and i3 t).
Higher velocity cells were taken for the inhibitor assay.
To calculate cell migration velocity, cells were tracked using the timelapse macro in Axiovision software.
All experiments were performed using Spin-Analytical sedimentation velocity cells with 1.2 cm centerpieces at 50K rpm.
Sample volume was 80-90 μL at concentrations of 5-500 nM in SedVel60K fluorescence velocity cells (Spin Analytical).
Finally, in contrast to linear phase coding populations, sigmoidal phase coding populations do not require additional information from head direction or velocity cells to generate directed theta sequences in open environments.
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Figure 10 Power along velocity dimension at target range cell, normalized by the amplitude of target velocity cell.
For velocity estimation, the cut-off value c is two times the size of the velocity cell.
Figure 9 Power along range dimension at target velocity cell, normalized by the amplitude of target range cell.
Similarly, Figure 10 depicts the power along the velocity dimension at the range of 1650 km, normalized with respect to the power of the velocity cell where the target exists.
Interestingly, we observed a reduction in cell velocity in cells expressing PIP4K2α (Fig. 3A), while expression of GFP alone in cells did not change cell velocity (Fig. 3B).
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