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In addition, it describes the flavonol regulator BvMYB12, being the first sugar beet R2R3-MYB with an experimentally proven function.
Genes claimed to encode serine glyoxylate aminotransferase and hydroxypyruvate reductase actually encode polypeptides with less than 30% identity to the respective enzymes with a proven function.
However, all these databases are neither specific for drought stress nor dedicated to genes with experimentally proven function under stress conditions.
Consequently, sites will be only mapped onto sequences if the site address matches such a defined sequence motif that is associated with known or proven function.
The functional classification was successfully verified by experimental confirmation of the prediction that the R2R3-MYB gene Bv_iogq encodes a flavonol regulator, thus making BvMYB12 the first sugar beet R2R3-MYB with an experimentally proven function.
Moreover, the MR from different species with proven function in RIA (S. cerevisiae Fre1-5 and S. pombe Frp1) or showing upregulation during iron starvation (C. neoformans Fre1 and A. nidulans FreA) are only distantly related.
Similar(51)
Firstly, similar genes have proven functions as toxins.
Ondex Web has been built as a Java applet, reusing proven functions and plugins from the Ondex desktop application.
It is worthwhile reminding that the GO annotations are suggestive of putative, rather than proven functions, as only a minority of Dictyostelium genes have been experimentally analyzed.
We first selected from Arabidopsis thaliana and Nicotiana benthamiana 30 genes with proven functions in the above mentioned categories, and the corresponding proteins were downloaded from NCBI [see Additional file 5].
To prove function, we have recently shown that an antifungal chitinase identified from Pseudotsuga drops exhibits enzyme activity both in situ and in vitro (Coulter et al., 2012).
Related(20)
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