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Since a majority of sequenced/annotated genes currently have no known (experimentally confirmed) function, the systematic locus ID also provides a useful way of tracking information about putative gene function.
Our attention focused on genes with previously confirmed function in G1 or G1 to S-phase transition.
The cells were kept in culture for more then 7 months and 58 passages, in absence of antibiotic selective pressure, and we confirmed function regularly by stimulating with agonists for endogenous GqPCRs (Gαq Protein Coupled Receptors), whose activation induce a Ca2+ release from internal stores through the Gαq/phospholipase C pathway.
Within this family, recent efforts to inhibit CDPK1 (Kato et al., 2008) could be complemented by CDPK5 as additional target, consistent with its recently confirmed function in P. falciparum schizont rupture (Dvorin et al., 2010).
Therefore, authors should provide a list of all sequences that were used as queries in these HHpred searches, explaining why they were chosen (e.g. experimentally confirmed function, structure available, was previously predicted to be a Cas protein, etc) and providing corresponding references.
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We implemented the proposed TM server on OpenStack Folsom, confirmed functions feasibilities and measured performances.
We identify 21 surface membrane factors, among which three have confirmed functions in HIV infection, seven have been identified by at least two other studies, and eleven are novel predictions and thus excellent targets for experimental investigation.
Remarkably, among these cell surface proteins, three have confirmed functions in HIV infection, seven have been reported by at least two other studies and eleven predictions are novel findings that deserve experimental investigation.
These results confirmed functions of 53BP1 in regulating EMT and invasion and migration of breast cancer at the clinical level.
Many of these miRNAs have confirmed functions in CRC development and some have been used as markers for predicting treatment response.
Regarding Linux patches, distributors such as RedHat confirm function degradation when they release a patch or upgraded version, and users can adopt a stable software version provided by distributors.
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