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The peak current during the anodic scan was found to reflect the amount of the target protein captured into the sandwich configuration.
The amount of protein captured by each rod was detected via a modified immunoenzyme assay using an anti-GSTP1 polyclonal antibody (Oxis Research, Portland, OR). at a 1∶2,000 dilution.
Furthermore, using a protein A-tagged Puf3p protein captured with IgG-Sepharose to conduct affinity chromatography, Gerber and colleagues characterized 220 target mRNAs that mainly encode mitochondrially-localized proteins [17].
In these assays the amount of light produced is proportional to the amount of soluble fusion protein captured, directly or indirectly by the antibody-bound beads.
Stromal cell-derived factor-1 is a small pro-inflammatory chemoattractant cytokine that was first cloned by Tashiro et al, 1993, as a protein captured by its specific amino-terminal signal sequences.
The interaction between BiP and NNV capsid protein was further confirmed by a co-immunoprecipitation assay, in which the capsid protein was co-precipitated with the BiP protein captured by anti-BiP antibodies.
Similar(48)
Having clearly demonstrated that Sn binds HIV-1, Sn could possibly perform dual functions, with some Sn protein capturing free HIV-1 while other Sn molecules would engage sialic acid conjugates on the target cell.
The results showed that the WCX magnetic beads could screen more proteins from serum samples of TB patients, with higher accuracy of the protein peaks and had stronger ability of protein capturing.
They activated only a few proteins, captured an image, and then activated some more proteins.
This will require assays designed to resolve the proteins captured by each binder.
Protein arrays for this study were made by expressing both the bait proteins (proteins captured at the surface) and prey proteins (probes) in cell-free rabbit reticulocyte lysate (RRL) systems.
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