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The capability of the prepared probe to detect metal ions was evaluated by the changes in its emission intensity.
The prepared probe was found to be an efficient and selective substrate of caspase-3, with Vmax of 41.4 ± 3.3 nM/min and KM of 1.60 ± 0.23 μM.
Different cations were investigated against the newly prepared probe and the results showed that Hg2+ was the one with the most fluorescence quenching ability.
The sensing platform (Au/Probe P) was prepared by placing 4 μL of freshly prepared Probe P (2 μM) solution on the Au electrode and then covering the end of the electrode with a plastic cap to prevent the solution from evaporating.
The sensing platform (Au/Probe ON-P1-P2) was prepared by placing 15 μL of freshly prepared Probe SH-ON-P1 (0.5 μM, in 200 mM Tris-HCl containing 5 μM TCEP, pH 7.4) solution on the Au electrode for 16 h at 25 °C.
Similar(54)
As shown in Fig. 4e, while the prepared probes incubated with ES CCE cells for 30 min, the prepared probes can be internalized into CCE stem cells.
Meanwhile, the MGC803 and GES-1 cells treated with the prepared probes were used as the control group.
Fig. 3 SEM images of the prepared probes (a and b) and diagram of the measurement scheme (c).
After successful conjugation, all prepared probes were stored at 4 °C until further use.
Repeat hybridisations were performed with freshly prepared probes and cross over of samples.
Freshly prepared probes were added to 5 ml of the same prehybridization solution, and hybridization continued overnight at 65°C.
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