Our immunoblot of native C. parvum and C. hominis antigenic preparations probed with C. parvum-specific patient sera revealed an apparently C. parvum-specific antigen of the expected size for cpCops-1 but not for ChCops-1.
Thylakoid membrane sample preparation, probe laser pulling parameters, and information on SECM instruments are available.
Chromosome preparation, probe hybridization and post hybridization washes were performed as described previously [ 35, 36].
Microarray slide preparation, probe labeling and hybridization and data analysis were described elsewhere with some modifications [ 38, 39].
The procedures for chromosome harvest, slide preparation, probe labeling, hybridization, and image capture were as described previously [ 37, 62- 65].
The tissue preparation, probe synthesis and hybridization were performed at the University of North Carolina at Chapel Hill Neuroscience Center Molecular Neuroscience Core Facility (http://www.med.unc.edu/neuroscience/core-facilities/molecular-neuroscience).
RNA preparation, probe synthesis, hybridisation, chemiluminescent detection, and image and data acquisition and analysis were performed according to the manufacture's protocol.
The cDNA preparation, probe labeling, hybridization and image analysis of the arrays were carried at ICBR Core Facility (UF) according to the manufacturer's recommendations (Affymetrix, Santa Clara, CA, USA), using Affymetrix Human Genome U133A GeneChip containing 47000 transcripts.
It should be noted, however, that array-based studies are limited by the quality of the target preparation, probe specificity and sensitivity, and for the Affymetrix platform we used the lack of interrogation of exons < 25 bp., and can be confounded by tumor tissue heterogeneity.
Total RNA sample preparation, cRNA probe preparation, array hybridization and data analysis were done as described previously [ 24].
