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All specimen preparation, tissue culture, and PCR testing was done in physically isolated rooms to prevent contamination.
Again, consistency in the tissue preparation, tissue sections and cells to be analysed is paramount.
In the phantom preparation, tissue absorption and scattering were simulated with India ink and Intralipid, respectively.
In situ hybridization can be divided into five phases: section preparation, tissue unmasking, hybridization, post-hybridization washes, and, detection.
Slide preparation: tissue was harvested and fixed immediately with 4% paraformaldehyde (Beijing CellChip Biotechnology Co., Ltd., Beijing, China) and 0.1 M PBS (PH 7.0 to 7.6 in 1/1000 DEPC (Sigma-Aldrich, St . Louis MO, USA).), followed by dehydration, paraffin embedding, and sectioning of a series of 6 slides with a thickness ranging from 4 to 6 μm.
After culture preparation, tissue viability (measured as ATP-content) and expression of fibrosis markers were determined using real-time PCR and immunohistochemistry. Slices remained viable up to 72 h of incubation and no significant effects of TGFβ1 and IFNγ on viability were observed.
Similar(52)
Microsurgical preparation of tissue explants avoids the destruction of the organ-specific tissue architecture.
Extracellular matrix (ECM) has drawn a broad attention for preparation of tissue engineering scaffolds and stem cell study.
Further preparation of tissue for EM is described below.
Preparation of tissue sections was carried out as described [2], [16].
The resulting tumours were collected for preparation of tissue lysates.
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