The preparation of substrate was made according to Standard EN 14780 – Methods of preparing biomass samples.
Preparation of substrate solutions: The assay substrates PheDU, PheHyd and IMH were dissolved in potassium phosphate buffer (0.1 M, pH 8) to a concentration of 4 mM assisted by heating to 70°C for 30 min.
The process of selecting coatings must consider the operating conditions, material compatibility issues, nature and surface preparation of substrate, time or speed of application, cost, safety, environmental effects, coating properties, and structural design.
An important aspect in the preparation of substrate materials to use in atomic force microscopy lies in the question of interactions introduced by treatments designed to immobilize the sample over the substrate.
We present results on modification of a focused ion beam based lift-out technique to meet the challenges for specimen preparation of substrate-free thin film material, which allows to avoid substrate interference during post-deposition annealing.
By a synergy effect of increasing number of HS-AFM users and concomitant accumulation of the know-how to conduct successful HS-AFM imaging (preparations of substrate surfaces and samples), the number of dynamic videos to be placed in future biochemistry textbooks will increase.
As the fabrication process of this NIR-SERS substrate is very simple and inexpensive, this method may be used in large-scale preparation of SERS substrates that have been widely applied in Raman analysis.
