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This diversity might be essential for regulating sequential processing of substrates.
This may endorse recognition, binding, and processing of substrates, ligands, as well as receptors and marks this domain as an additional target for the design of selective regulators.
The ClpA molecule generated in this way lacks the N-domain and the associated linker but is impaired only slightly in the processing of substrates that are degraded independently of ClpS.
These data highlight the sensitivity of the methods employed in this study, as we were able to detect BACE1-dependent processing of substrates expressed at undetectable levels in cellular lysates.
Therefore, further work is needed to delineate whether additional interactions within the bacterial cell cytoplasm are necessary for optimal autocatalysis or processing of substrates by PepD in the extracytoplasmic space.
Others have fabricated related devices using femtosecond laser processing of substrates, primarily towards cell counting or sorting applications.
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The phenotypic changes that were found in MMP19-deficient mice could be rather a result of combined processing of multiple substrates than outcome of one major substrate.
Interestingly, while WRN ATPase and helicase activities do not influence processing of telomeric substrates, WRN exonuclease on non-telomeric 3' overhang substrates is stimulated by ATP hydrolysis [ 17].
In vitro processing of semisynthetic substrates unrelated to the prelacticin 481 peptide demonstrated the broad substrate tolerance of LctM.
The γ-secretase processing of the substrates was monitored by comparing the levels of formed ICDs from the different substrates using western blot analysis.
The processing of such substrates in reducing hydrogen atmosphere results in the formation of the metal nanoislands on the substrate surface.
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