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Germ cells were quantified by screening for cells undergoing cellular division (as assessed via DNA morphology), and then assessing the percentage of dividing cells which positively express both STELLA and VASA.
This is particularly important in the treatment of a tumor, as even if <100% of the cancer cells are transfected in vivo, the fraction of cancer cells that positively express HSVtk can lead to the apoptosis of neighboring untransfected tumor cells.
It's very attractive to a man that you know your feelings and can positively express them.[18].[18]
Once you have figured out, somewhat, what you are feeling, ask yourself "where in my body is this feeling?" "What is the shape and texture of this feeling?" "Does this feeling have a color?" Giving concrete form to your feelings, helps you accurately and positively express them.
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Stromal CXCL1 protein expression was positively expressed in all tumors examined, while the RNA was expressed in a small subset of breast tumor samples.
For the positive control, a foregone positively expressed tissue section was used.
TIP30 is positively expressed in many normal human tissues but decreased expression is observed in some tumour tissues such as melanoma, breast cancer, neuroblastoma, glioblastoma, colon cancer and hepatocellular carcinoma (HCC).
Cystatin A was positively expressed in 62.3% (71/114) of tumor tissues, and expression was associated with age (P = 0.009; Table 1), but not with sex, pathologic type, or stage (P > 0.05; Table 1).
Sequences with a level of expression of 200 and over were considered to be positively expressed.
While stromal CXCL1 was positively expressed in all groups, there were no significant differences in expression among the subtypes in either DCIS or IDC patient samples (Additional file 1: Figure S1 and Additional file 2: Figure S2).
Positively expressing cells were validated using light intensity cutoffs relative to positive and negative control cell samples.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com