Stromal CXCL1 protein expression was positively expressed in all tumors examined, while the RNA was expressed in a small subset of breast tumor samples.
For the positive control, a foregone positively expressed tissue section was used.
TIP30 is positively expressed in many normal human tissues but decreased expression is observed in some tumour tissues such as melanoma, breast cancer, neuroblastoma, glioblastoma, colon cancer and hepatocellular carcinoma (HCC).
Cystatin A was positively expressed in 62.3% (71/114) of tumor tissues, and expression was associated with age (P = 0.009; Table 1), but not with sex, pathologic type, or stage (P > 0.05; Table 1).
While stromal CXCL1 was positively expressed in all groups, there were no significant differences in expression among the subtypes in either DCIS or IDC patient samples (Additional file 1: Figure S1 and Additional file 2: Figure S2).
Sequences with a level of expression of 200 and over were considered to be positively expressed.
ECs positively expressed VEGFR2, VE-cadherin, and PECAM-1, while SMCs were positive for calponin, SMA-α, and MYH-11).
EqMSCs positively expressed the undifferentiated pluripotent mesenchymal stem cell surface markers CD44 and CD90.
MSC differentiation results showed that induced osteoblasts positively expressed alkaline phosphatase as shown in Figure 1g.
Moreover, western blot also indicated that all hygromycin survival progenies positively expressed eGFP protein (Fig. 5d).
As shown in Figure 1b,c, immunofluorescence staining results showed that passage 3 MSCs positively expressed CD29 and CD90 antigens.
