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The MLVA assay developed in this investigation showed to be a reproducible and portable assay which could be easily performed and transferred to other laboratories.
This article describes the development of a simple, portable assay system using microfluidic paper-based analytical devices (μPADs) coupled with colorimetric detection for rapid measurements.
Biological assays to measure DNA and RNA concentrations are readily available in a laboratory environment, but are not yet available in a portable assay format suitable for use in home-based diagnostics or point-of-care diagnostics in resource poor settings.
A rapid, highly sensitive, and portable assay for clinical laboratories and field use is required.
Similar(56)
Thus, high throughput laboratory assays and rugged field portable assays are needed.
This is possibly because of a paucity of convenient and portable assays for monitoring enzyme activity, and of the difficulty of obtaining many of the protein components and complexes in quantities suitable to detailed biophysical characterization.
A field-portable, assay-specific instrument has been developed based on this methodology.
Importantly, this portable field assay allows for the economic usage of limited biological material (parasites and reticulocytes) and the intracellular labeling of the target cells reduces the need for highly purified schizont inoculums.
Ideally, a portable qPCR assay would only require measurements on a single undiluted sample.
This paper develops a simple, inexpensive, and portable diagnostic assays that may be useful in remote settings, and in particular, in less industrialized countries where simple assays are becoming increasingly important for detecting disease and monitoring health.
These methods suggest a path for the development of simple, inexpensive, and portable diagnostic assays that can be useful in remote settings, where simple immunoassays are becoming increasingly important for detecting disease and monitoring health.
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