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Other approaches include LMD (Mobile Assay, CO, USA), whom use a custom software application running on a cell phone.
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In addition, the mobile zinc assay is much faster than current zinc AAS technology.
Using HcZ9, a high-throughput mobile zinc assay was developed with comparable accuracy to zinc AAS.
The mobile zinc assay, however, offers several advantages over zinc AAS.
Electrophoresis mobile shift assay was performed with nuclear extracts from human HEK293T and SH-SY5Y cell line.
For high-throughput analysis, the HcZ9-based mobile zinc assay was developed in a standard 96-well microplate format.
Both techniques yielded similar results, indicating that the mobile zinc assay and zinc AAS have comparable accuracy.
After validating the mobile zinc assay in cell lysates, we examined the relationship between total zinc and mobile zinc in prostatic cells.
Given its facile nature and accuracy, however, our mobile zinc assay should prove useful in studying these levels in complex fluids or tissues at the cellular level.
Details regarding the synthesis and characterization of HcZ9, as well as additional experiments, methods, and a detailed protocol for the mobile zinc assay are available free of charge via the Internet at http://pubs.acs.org.org
A mobile zinc assay using HcZ9 was developed that is compatible with standard fluorescence plate readers and quantifies zinc with accuracy comparable to that of zinc atomic absorption spectroscopy (AAS).
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