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Exact(7)

Quantification of the PLA data confirmed specific PLA labeling for reactions with the GCC185 N- and C-terminal domain antibodies, consistent with proximity of the N- and C-termini of this protein on the Golgi in cells.

In particular, there are concerns about the interpretation of the PLA data and how well the PLA analysis measures distance between the two BCR subunits, and whether your results mean that the BCR dimers actually dissociate or rather reorient and/or undergo a conformational rearrangement.

DOI: http://dx.doi.org/10.7554/eLife.02069.003 The PLA data were quantified by the BlobFinder software (Allalou and Wahlby, 2009) and showed that the Fab-PLA differences between resting and activated B cells are statistically significant.

MKM was involved in experimental design of the PLA, data evaluation and writing the manuscript.

The PLA data suggest that IQGAP1 and caveolin-1 both act as ERK1/2 scaffolds in the same phorbol ester induced ERK1/2 activation pathway.

Ideally, the authors should back-up their PLA data with data generated by a completely different technique, such as EM or native gels.

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Similar(53)

We validated the specificity of this approach by setting up experimental conditions in MCF-7 cells and confirmed by PLA the in vitro data on the formation and regulation of the complex ERα/Src/PI3K (Figs 1 and 2, Supporting Information Figs S1 S4).

In agreement with data from PLA experiments, ISO treatment triggered translocation of both PKD1 and Hsp20 to the nucleus that could be attenuated with the disruptor peptide but not with control.

To complete phase I, the university researchers engaged in 'matched' data-generation (PLA style interviews: n=4; PLA style focus groups: n=2) for literature-sharing and strategy-mapping activities with GPs, practice receptionist, service planners and formal trained interpreters eliciting their perspectives on communication in cross-cultural general practice consultations.

We used researchers' debriefing notes and meeting minutes to augment the data recorded on PLA charts after all data generation sessions.

These supervision and inter-rater agreement procedures have been proven effective in our previous studies and generated excellent diagnostic reliability for PLA. 3 34 Participants' sociodemographic data will be presented in descriptive form.

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