Sentence examples for patch solution from inspiring English sources

In perforated-patch experiments reported in Figure 3A, pipettes were tip-filled with patch solution containing ≤0.5 mg/mL amphotericin B (1% DMSO; Sigma).

Micropipettes were backfilled with internal patch solution containing (in mM) K-gluconate 110, KCl 10, HEPES 10, Phosphocreatine 10, Mg-ATP 4, Na-GTP 0.3, Biocytin 0.1%, pH 7.3, with and osmolarity of 280 290 mOsm.

Current-clamped recordings were obtained with the above internal patch solution consisting of 135 mM K-gluconate.

Some cones were recorded with an EGTA zero Ca 2+ perforated patch solution in the pipette (blue lines; see 'Results').

Biocytin (0.5 mg) was mixed into 1 ml of patch solution just before use and filtered as used.

For pharmacological experiments, acetazolamide (ACTZ, Sigma-Aldrich) was first dissolved in dimethylsulfoxide (DMSO) then in the external patch solution at the desired final concentration.

The proposed approach retains the high level of accuracy of single patch solutions and reveals its potential for authentic multi-patch NURBS modeling.

In fact, Rusovsky says the development of Rox was influenced by feedback from its existing customer base, who had manipulated the hot patching solution to deploy new features safer and faster.

In contrast, in the two-patch solution, β T is set to 0.2 per year, with the other two-patch parameters the same as the analogous four-patch parameter values.

The standard patch pipette solution contained (mM): 145 NaCl, 1.8 CaCl2, 2 MgCl2, 5.5 glucose, and 10 HEPES, pH 7.4 with NaOH; with this solution, patch pipette resistances were 10 15 MΩ.

Inclusion of 100 µM dipyridamole in the patch pipette solution also prevented deoxygenation-induced activation of SS red cell membrane patch cation conductance.