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When peptide sequences could not be obtained unambiguously by using ESI-MS/MS, Edman degradation was performed using an Applied Biosystems Procise cLC sequencer to obtain sequence information for protein identification.
Images acquired from the Illumina sequencer were processed through the Illumina pipeline to obtain sequence and quality scores for each base.
After, we obtain sequence patterns from these extracted itemsets.
We obtain sequence u k m r ⊂ u k m r - 1, u p ∉ u k m r and there exists u r ∈ E r such that ∥ u k m r - u r ∥ r → 0, k m → ∞, r = 1, 2, ….
Ecotoxicological literature examples demonstrate that, under strictly standardized conditions, the RAPD technique can be a useful tool to preliminary assess toxicological population genetic effects, particularly since this technique is relatively inexpensive and yields information on a large number of loci without having to obtain sequence data for primer design.
We then isolated DNA from the mixtures and used two approaches to obtain sequence data.
We therefore sequenced the tumor and normal genomes of patient 8, to obtain sequence coverage of ∼0.8x (Methods).
This type of pyrosequencing would allow us to obtain sequence results of similarly high quality in 10 to 15 minutes (Figure 5).
The sequences of each group were aligned to obtain sequence logo of the translation initiation site using WebLogo 3, version 2.8.2 (http://weblogo.berkeley.edu/).edu/
CAGE is a technique to obtain sequence tags (CAGE tags) of about 20 nucleotides from 5'-ends of capped transcripts [55].
The VEM method, consisting of viral purification and metagenomic sequencing, was used to obtain sequence from DNA present inside virions in the whitefly vector, Bemisia tabaci.
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