The phrase "attain sequence" is not commonly used in written English and may not convey a clear meaning.
It could potentially be used in contexts related to achieving a specific order or arrangement, but it lacks clarity without additional context.
Example: "In order to complete the project successfully, we need to attain sequence in our tasks."
Alternatives: "achieve order" or "establish sequence".
d sORFs with mapped ribosome profiles, attaining sequence read coverage > 75% of the total ORF (based on cycloheximide treatment), and ribosome profile hits at the ORF start site (based on harringtonine treated samples).
These common issues in metagenomics might be overcome by switching either to a longer read sequencing technology or by more sequencing to increase coverage (i.e. the number of calls of a base in a given DNA sequence, typically attained by sequencing multiple molecules containing the respective sequence), but in any case they mean increasing costs, data volumes and complexity.
However, the future of genetic analysis in autoimmune diseases and its corresponding applications in treatment, healthcare, and population studies lie in the rapid progress of next generation sequencing platforms which in the very next years will attain the sequencing of whole exome, transcriptome or genome, in a low-cost and accurate manner.
KEGG Orthology contains to date more than 1 million sequences from nearly 1,000 genomes and it was enriched by a procedure developed by our group to attain 2,442,384 sequences from 25,024 organisms, constituting the UEKO database (UniRef50 enriched KEGG Orthology database, to be published elsewhere and further distributed).
In order to attain the sequences with high quality, all reads with adaptor contamination were discarded.
Sequences not attaining the 8× sequence coverage threshold were excluded from further analysis, as were putative high-copy RAD sequences where the number of sequence occurrences in each cluster was greater than 500 (500×).
Only where we could attain good quality sequence data for variable domains II and IV of ompA did we accept the identification data as accurate.
For example, Campbell et al (2008) used Illumina GA technology to attain the precise sequence of several hundred variants, comprising germline and somatic intra- and inter-chromosomal rearrangements in two lung cancer cell lines.
An analysis based on the rate at which background signal is converted to foreground signal shows that we have attained sufficient sequencing depth for genome coverage (data not shown).
Interrogation of large genomic rearrangements in DNA from FFPE tissues currently requires whole genome sequencing of PE libraries on several lanes of an Illumina HiSeq2000 flow cell in order to attain high enough sequencing depths to confidently call fusion breakpoints.
