For decades the major obstacle for studies into the roles of astrocytes was the lack of tools suitable for selective manipulation of these cells, in situ, in the context of intact brain tissue.
Due to the rapid development time of Ciona embryos (16 hr to hatching, 24 hr to onset of metamorphosis), this transience is not really an obstacle for studies focused on the embryo or larva.
A potential obstacle for students studying natural selection is a lack of appreciation for the extent and significance of variation within populations.
One major obstacle in implementing electrochemistry for studying biomolecular reaction is its inadequate sensitivity.
As no protein-coding genes, even those that are universally distributed, are highly conserved in its DNA sequence, the design of low-redundancy primers that allow amplification of homologous regions from divergent taxa has been the major obstacle in their application for studying microbiomes.
Moreover, for studying materials containing high obstacle densities, no significant influence of the FIB damage is observed.
The scarcity of polyadenylated RNAs is one of the obstacles for investigators studying bacterial polyadenylation.
