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These difficulties generate a limitation for studying the functions of TuMV mutants, in which weaker infectivity may be incurred by mutations.
Despite this inherent limitation for studying mechanisms underlying tinnitus perception, deficits in the gap-prepulse inhibition of the acoustic startle reflex (gap detection) is the most widely used behavioral test for assessing behavioral evidence of tinnitus in animals (Galazyuk and Hebert, 2015).
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In vivo animal models have intrinsic limitations for studying relationships between tuberculosis and its host and there is a need for alternative, in vitro cellular models.
Although useful for probing bacterial pathogenesis and physiology, current random mutagenesis systems suffer limitations for studying the toxin-producing bacterium Clostridium perfringens.
As well as the advantages listed above, randomised studies tend to have some limitations for studying late effects.
Considering that the cephalometric measurements have some limitations for studying the morphology of craniofacial structures, it is necessary to investigate the craniofacial morphology through three-dimensional analyses which allows the inheritance of these structures in different malocclusions to be identified.
However they did not examine cold effects in detail and did not consider lags beyond 2 days, a serious limitation for studies of cold effects on mortality.
Our analyses showed that the SNP databases in their current status might have some limitation for studies of complex disorders, especially in different ethnic groups, as a result of incomplete or uneven representation of SNPs along the genome [ 23].
This study also highlights the capabilities and limitations of nanoindentation for studying the anisotropic mechanical properties of hybrid framework materials.
Our model addresses the major limitations of relevant models for studying image watermarking schemes.
Throughout the discussion we will also point out the limitations of GPS techniques for studying disturbances.
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