Sentence examples for nucleus fraction from inspiring English sources

aCyclin D1 overexpression with nucleus fraction ≥50%.

bCyclin E overexpression with nucleus fraction ≥50%.

At the lowest concentration of vector, KLF15 is mainly over-expressed in the nucleus fraction.

Broken cells were centrifugated (1,000 × g, 4°C, 10 min) to collect a crude nucleus fraction.

The increase of NF- κB/p65 in cytosol corresponded to the decrease in nucleus fraction, indicating the decrease of NF- κB translocation in RCAN1 transfected cells.

It was then centrifuged at 7000 × g at 4°C for 10 min, and the supernatant was used as the nucleus fraction.

In addition, to determine whether the altered level of β-catenin phosphorylation affects the subcellular localization of β-catenin, NP-40 soluble (cytosol) and insoluble (nucleus) fractions were extracted from C2 and N20 cells.

Western blot analysis showed that treatment of MDA-MB-231-ER:FOXO3a(A3) cells with 4-OHT induced the relocation of FOXO3a from the cytoplasm to the nucleus fractions (Fig. 10A).

To assess whether acute high-dose morphine induces CREB activation and whether co-treatment with 100 pM NLX, NTX or the FLNA pentapeptide alter it, the level of protein kinase A-phosphorylated CREB, pS133-CREB, was assessed in cytosolic and nucleus fractions generated as described above from striatal slices.

To examine RelA translocation to the nucleus, we used a subcellular proteome extraction kit (S-PEK; Calbiochem, Darmstadt, Germany) according to the manufacturer's instructions to extract cytoplasm, cell membrane and nucleus fractions of HSG cells.

In this procedure, COX-2 is mainly located in the non-nucleus fraction, which was used for the present assay.