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The post nuclear fraction is passed through the magnetic field.
Further, nuclear fraction and unbroken cells are separated from post nuclear fraction by centrifuging at 800 rpm for 10 min at 4 °C.
The post nuclear fraction is passed on the column in presence of magnetic field.
Fibrillarin was used to assess the purity of the nuclear fraction.
Supernatants constituted the nuclear fraction.
The supernatant is the nuclear fraction.
The supernatant was collected as nuclear fraction.
The pellet contained the nuclear fraction.
The pellet (P1) contained cell membranes and the nuclear fraction.
The supernatant was saved as the nuclear fraction.
As anticipated, we observed an increase in phospho-STAT3 in the nuclear fraction following PAF stimulation but not in the unstimulated control nuclear fraction (Figure 6k).
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