Sentence examples for nuclei fraction from inspiring English sources

This suspension represents the "shielded" nuclei fraction.

The crude nuclei fraction was pelleted by microcentrifugation at 5000 rpm for 5 minutes at 4°C.

The pellet, referred to as the nuclei fraction, was suspended in 1 ml buffer C (50 mM TES pH 7.4, 50 mM sucrose, 1 mM EDTA, 0.1 mM EGTA, 1 µg/ml pepstatin A, 10 µg/ml leupeptin and 10 µg/ml antipain).

The nuclei fraction was fractioned at 800 × g for 10 min.

The Ki67 expressing nuclei fraction ranged from 42 to 68% over this time frame.

Along the apical- to basal heart axis, the CM nuclei fraction in the ventricles did not differ significantly (Fig.  5d).

The CM nuclei fractions were quantified by counting H2B-mCh+ nuclei and total number of nuclei.

The nuclei fractions were prepared as described in the Materials and Methods Section.

To quantitate target protein expressions in each cellular compartment, whole cell lysate, membrane, cytosolic, and nuclei fractions were prepared using differential centrifugation as we previously described [ 24].

Thirty micrograms of proteins from the cell or nuclei fractions were separated by SDS-polyacrylamide gel electrophoresis using 10% polyacrylamide gel.

To confirm the enrichment of the fraction, anti-Na+-K+-ATPase and anti-lamin B1 antibodies were used as membrane and nuclei fractions, respectively, similarly to previously described [ 31, 32].