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Figure 2a shows small nuclei centers on the nanofibers after 10 min of reaction time.
Based on the Voronoi diagram of the nuclei centers, it accounts for the curvature of the grains near triple junctions.
A large number of nanoparticles with diameters of about 30 nm can be clearly observed on the surfaces of these microspheres, which will serve as the nuclei centers for the growth of γ-MnS nanorods in the next step.
However, the existing principal curve detection methods (Hastie, 1994; Kégl et al., 2000) cannot be employed directly, because worm cells have varying morphologies, intensities and sizes, and so cannot be simplified to a set of points such as the nuclei centers.
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Strain was calculated by nuclei displacement (geometric center) from their coordinates imaged at 5 mmHg (reference state).
Under in vitro, simulated conditions, the microtubule seeds also probe the metallic boundaries of artificial chambers with cellular dimensions to bring nuclei to the center [ 16].
During skeletal muscle development, nuclei move toward the center of the myotube after myoblast fusion and spread along the center of the myotube prior to positioning at the periphery of the myofiber (Cadot et al, 2012; Metzger et al, 2012).
The nuclei cluster in the center of the tube is pointed out by anarrow in (f) and (g).
Additionally, not all cells are flat nor are their nuclei positioned in the center of the cell.
We observed that unlike wild type white adipocytes that contain a large unilocular lipid droplet, Fsp27−/− white adipocytes have small but numerous lipid droplets in their cytosol, with nuclei localize at the center of the cell.
In parallel, nuclei move from the center of the myofiber to the periphery (Franzini-Armstrong, 1986; Flucher et al, 1991; Starr & Fridolfsson, 2010; Metzger et al, 2012).
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