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Previous TKA designs have not fully replicated normal knee motion, and they are characterized by lower magnitudes of overall rollback and axial rotation than the normal knee.
At each cellular division, telomeric DNA terminal regions are not fully replicated, which, if not counteracted by elongation by telomerase, can lead to telomere shortening.
However, in recent phase I/II trials and one unpublished phase III trial, these findings were not fully replicated.
These observations, demonstrating that the LmcDNA16 null parasites can undergo metacyclogenesis in vitro but not in vivo, suggest either that the metacyclic phenotype observed in the vector and required for parasite transmission in vivo is not fully replicated in vitro or that other factors are critical for differentiation in the sand fly.
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While in vitro work does not fully replicate the natural environment, it does allow the study of isolated factors.
Similarly, while it appears probable that these data clarify the potential mechanisms of SPA-induced lung injury, these experimental models do not fully replicate the biochemical or physiological milieu found in porcine or human lungs.
Existing in vitro methods for testing denture adhesives do not fully replicate the complex oral geometries and environment; and in vivo methods are qualitative, prone to bias and not easily reproducible.
Although several advancements have been achieved in the field of cardiac tissue engineering, current in vitro cardiac tissues do not fully replicate the structure or function necessary for effective cardiac therapy and cardiotoxicity studies.
A broader and more porous/permeable reaction zone was created in the laboratory experiments compared to the natural samples, and it is possible that short-term experiments might not fully replicate slower, longer-term processes.
When nucleosomes are assembled in vitro, in the absence of other factors, the positions adopted by nucleosomes do not fully replicate those observed in vivo [ 63,64].
Further, FCMs do not fully replicate the mirco-level interactions that may prove to be powerful in determining meso- and macro-level outcomes.
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