Sentence examples for neutralizing buffer from inspiring English sources

The eluted samples were neutralized immediately with a 30 μl of neutralizing buffer (1 M Tris-HCl, 2 M NaCl, pH 8.8).

Following stimulation, T cells were harvested and washed three times in RPMI 1640 prior to fixation for 1 minute in PBS containing 0.05% glutaraldehyde, and were than neutralized with an equivalent volume of T-cell neutralizing buffer containing 0.2 M glycine.

After electrophoresis, slides were drained and washed thrice with neutralizing buffer (0.4 M Tris-HCl, pH 7.4) for 5 min each.

The colour development reaction was stopped in neutralizing buffer (0.01 M Trizma base and 0.001 M EDTA, pH 8).

Cells were re-suspended in stripping buffer (0.13 M citric acid, 0.06 M sodium phosphate monobasic, pH 3.0, 1% BSA) for 120 seconds on ice, then five volumes of neutralizing buffer were added (0.15 M sodium phosphate monobasic, 1% BSA, pH 7.5) before pelleting cells for 10' at 4 C at 1,200 rpm.

The slides were incubated 2 h at 4°C in lysis solution (2.5 M NaCl, 100 mM EDTA, 10 mM Tris, pH 10, 10% DMSO, 1% Triton X-100) followed by 3 washes in neutralizing buffer (0.4 M Tris-HCl, pH 7.5).

Phenol red added to neutralizing PBS buffer colored the PBSA hydrogels red over 3 days and serve as a visual marker for bulk hydrogel pH.

The four dietary treatments were formulated to be isonitrogenous, iso-starch, and to differ in the main starch source in the concentrate and the addition of buffers and neutralizing substances (B: sodium bicarbonate and magnesium oxide).

Small airway epithelial basal medium (SABM), and small airway epithelial growth media (SAGM™) bulletkit; and Reagentpack™ containing Trypsin 0.025%/EDTA 0.01%, Trypsin neutralizing solution and HEPES buffered-saline solution were purchased from Lonza Walkersvillle Inc. (Walkersville, MD).

Small airway epithelial basal medium (SABM), and small airway epithelial growth media (SAGM™) bulletkit; and one Reagentpack™ containing Trypsin 0.025%/EDTA 0.01%, Trypsin neutralizing solution and HEPES buffered saline solution were purchaged from Cambrex Bio Sciences Walkersvillle, Inc. (Walkersville, MD).

Thus, it is possible that the buffers, by neutralizing tumor acidosis, are decreasing the proteolytic enzyme activation and prevent the ECM degradation which leads to decrease metastasis.