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This method efficiently captures and compares the similarities in the phylogenetic topologies produced by individual gene trees with no need of a reference species tree.
These data, combined with a unique custom analysis approach, eliminate the need of a reference strain, which previously has been an absolute requirement for accurate comparative genomic hybridization (CGH) studies.
The important difference from the known structure-analyzing tools of GROMACS, whether based on RMS deviation after fitting or RMS deviation of atom-pair distances, is that there is no need of a reference structure here.
A major challenge for determining the microbial species present in a sample without the need of a reference database is to use algorithms other than those also used by the methods that depend on a reference database.
In recent years, two main categories of transcriptome reconstruction software tools have been developed: the reference-based software, that rely on reads previously aligned on a reference genome, and the "de-novo" assemblers that analyze RNA-seq reads without the need of a reference genome.
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These issues illustrate the value of combining different sequencing strategies to give a more comprehensive survey of the chromosome, and highlight the need for a reference sequence assembly of the entire wheat genome, which has so far only been achieved for 3B [ 17].
There is no need for a reference standard of the same chemical structure as the sample, as it is the case in chromatography or other analytical methods.
Conventionally, features such as harmonics-to-noise ratio [27] and preprocessing methods like database normalization involving speaker, corpus, language, or gender are used to cope with the need of defining a reference criteria [14] in emotion detection.
Square wave voltammetry was used to determine the oxidation potentials of the polymer bound redox species allowing the precise determination of the pH value of the electrolyte solution without the need of a stable reference electrode.
High quality empirical research in medical ethics is in need of a considered reference to normative analysis.
In this paper, we will argue that high quality empirical research in medical ethics is in need of a considered reference to normative research questions.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com