Sentence examples for mutations on membrane from inspiring English sources

The effect of these mutations on membrane binding was determined by a quantitative phospholipid ELISA assay and compared to wild-type α-Syn and to the Parkinson's disease-causing mutations, A30P, E46K and A53T.

Next, we tested the effect of these mutations on membrane binding.

Thus, we assessed the impact of the endophilin mutations on membrane fission in vitro using our biochemical vesiculation assay.

To assess the net impact of Rab7 mutations on membrane cycling, we used dynamic live-cell imaging to characterize wild-type and mutant GFP Rab7 in living cells.

The effects of Ras mutations on membrane orientation and effector binding were previously inferred by Abankwa and Hancock using indirect assays and molecular dynamics simulations.

The proposed mechanism agrees well with the experimentally observed features of EmrE topogenesis and provides a range of experimentally testable predictions regarding the effect of translocon mutations on membrane protein topogenesis.

We measured the effect of these mutations on membrane-binding using a lipid flotation assay, and on v-vesicle clustering using single vesicle microscopy.

Our experiments give further indications of the specific impact of the Kv1.1 channel mutation on membrane electrophysiology of β-cells from mceph/mceph mice.

Moreover, the C2 domain mutant form of t-PTEN (D5 t-PTEN, which contains mutations on the membrane binding CBRIII loop) was resistant to the catalytic inhibition effects of C-tail phosphopeptide, consistent with the model that a principal functional interaction between the body and phospho-tail involves the lipid binding interface of the C2 domain.

The effects of different Tecta mutations on the tectorial membrane have been analysed in detail, but less is known about Tecta function in the mammalian vestibular system, where it is also expressed (Rau et al., 1999; Goodyear and Richardson, 2002).

This observation is in accordance with the compromising effects of disease-related mutations in BAR domains on membrane deformation and further supports the role of full-length BIN1 in T-tubule biogenesis in vivo.