Sentence examples for mutation was checked from inspiring English sources

Transformants were selected at 30°C on phleomycin (Pm) containing plates (the phleomycin gene - pmr - is located just upstream of dnaE) and the presence of the dna(Ts) mutation was checked by analyzing cell growth and filamentation at restrictive temperature by plating and optical microscopy analysis.

Each published mutation was checked for accuracy by comparison to the GNAS wild-type sequence.

All sequences were inspected individually using the raw spectrograph data and every point mutation was checked for authenticity.

The vector expressing the NefG3C-GFP mutant was generated from the pcNefsg25GFP vector using the quickchange kit (Stratagene, La Jolla, CA), and the mutation was checked by sequencing.

Presence of the intended mutation was checked in homozygous LckS59Amice by sequencing a 434-bp genomic fragment encompassing the targeted chromosomal region and using primers 5′-GAATTCAGAGACAAGGTTCAACCA-3′ and 5′-AAGTCTCCATCATGGGAGGGCTCA-3′.

The cooperativity of mutations was checked by comparing the changes of experimental binding affinities (ΔΔ G) for the seven mutants in the series.

Mutations were checked by reference to the Leiden Muscular Dystrophy pages DMD gene reading frame-checker (http://www.dmd.nl/) and analysis with the online sequence variant checking software Mutalyzer (http://eu.liacs.nl/mutalyzer/).nl/mutalyzer/

All the mutations were checked also in available family members.

All the constructs and site-directed mutations were checked by sequencing using the ABI-Prism kit from Applied Biosystems.

The G1 mutations were checked for the translucent skin phenotype on the 3rd instar, and all the positive mutations were allowed to develop to the moths.

The codon alignment sequences that contain frameshift mutations were checked and edited manually in the software MEGA4 [ 23] if identity is low.