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A 4 base pair deletion and a point mutation producing a stop codon were identified in 1 cell line.
It is possible that its TMDs are missing as a result of a mutation producing a protein truncated at the N-terminus.
In humans, an 'Arctic' form of AD, caused by an APP mutation producing a Glu22 → Gly amino acid substitution in Aβ, appears to be a disease caused by protofibrils, the immediate precursors of fibrils [ 34- 36].
As points of reference we use existing endogenous alleles, in particular the strong fz P21 allele that harbors a nonsense mutation producing a early truncation of the protein and the cell-autonomous fz J22 allele that has the P278L substitution in ICL1 that is known to block Dsh recruitment to junctions (Jones et al. 1996; Axelrod 2001).
Similar(56)
Additionally, a Q672K mutation produced a dramatic slowing of activation kinetics, a phenomenon also observed when mutating the corresponding residue in mammalian TMEM16F (Yang et al., 2012).
Then with the help of selection, crossover, and mutation produces a new population.
The Gly28 Ser mutation produces a dramatic four-fold increase in its kcat and a remarkable increase in its stability.
Thus, the mutation of Ghd10 did not affect the growth rate of the mutant, but the mutation produced a gain-of-function in inflorescence development.
When a mutation produces a stop codon in the wrong place, it can lead to truncated, and thus worthless, protein.
Further experiments revealed that each mutation produced a more stable version of the energy metabolism protein, with the winner making the most stable one.
In this work, we demonstrate that a synthetic gene encoding LanFP10-A, a natural protein encoded in the genome of the lancelet Branchiostoma floridae containing the G67A mutation, produces a heterologous, functional yellow fluorescent protein when expressed in E. coli.
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