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Exact(6)
The deletion of these insertions is now shown to permit mutation of position 67, thereby increasing the primary structure symmetry relationship within the core.
In the framework of this challenge, this study focused on the family GH51 α-l-arabinofuranosidase from Thermobacillus xylanilyticus, with the aim to understand why the mutation of position 344 provokes a significant modification of the transglycosylation/hydrolysis partition.
Alanine mutation of position 9 (key residue 3) has no strong effect.
KanR clones were screened using PCR and sequence verified for mutation of position 246 to Ala.
Mutation of position 46 can abolish phosphorylation by HIPK2 and acetylation of K-382 by CREBBP [ 84].
In previous work, an ApPDC variant with a mutation of position 469 from glutamate (E) to glycine (G) was successfully prepared in order to generate an S-selective enzyme for the production of (S -PAC and derivativeS -PACeof.
Similar(54)
Mutation of positions 1 and 10 (key residues 1 and 4) to alanine abolishes cleavage.
Because all reported human nullomers contain multiple CpGs, their absence from the human genome may be caused by the hyper mutation of positions involved in CpG dinucleotides.
Interestingly, mutagenesis studies of the gene end sequences in RSV have shown that mutations of position five and six to nucleotides other than adenosine resulted in a strong decrease in antitermination efficiency (Harmon et al., 2001).
Mutation of this position has fewer effects on AM2 potency at both receptors (Qi et al., 2011).
Also, mutation of this position in AHL lactonase to an asparagine results in a significant decrease in kcat values.
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