Sentence examples for mutagenesis of position from inspiring English sources

However, saturation mutagenesis of position 10 identified one double mutant T210G/I10C with 100-fold higher specific activity with indene 1,2-oxide than wild-type GST M2-2.

The saturated mutagenesis of position N367 in HXT36 was done using PCR with Phusion® High-Fidelity PCR Master Mix with HF Buffer using primer pairs F HXT36 BcuI/R HXT36 367NNN and F HXT36 367NNN/R HXT36 BamHI (Additional file 1: Table S4).

Saturated mutagenesis of position N366 in Hxt11 was done by PCR using Phusion® High-Fidelity PCR Master Mix with HF buffer using primer pairs F HXT11 XbaI/R HXT11 366NNN and F HXT11 366NNN/R HXT11 BamHI (Additional file 2: Table S6).

Mutagenesis of positions 166 and 182 in CesA yielded novel variants with enhanced enantioselectivity and without significant loss of catalytic activity.

Site-directed mutagenesis of RNase H positions N474, Q475 and Y501 rendered the mutant enzymes inefficient in the removal of the PPT primer and modified the RNase H cleavage specificity [23].

To test this hypothesis, site-directed mutagenesis of these positions was performed in the CTX-M-14 β-lactamase.

Residues directly participating in the actin-binding site have been identified by site-directed mutagenesis of selected positions conserved among Bni1p and metazoan formins of the Diaphanous type.

We performed saturation mutagenesis of mTFP1 at position 163 and screened the library using a colony-based fluorescence imaging system.

Therefore, mutagenesis of ATGs at position 1 and 117 should not affect the protein expression and function.

Overall, these data demonstrate that the mutagenesis of the N366 position of Hxt11 yields mutants that as a single transporter mediates a balanced uptake of glucose and xylose, thereby supporting co-consumption of the these sugars at overall sugar conversion rates that are in line with the rates observed in a strain using the full complement of native transporters.

Using the Na+/proline transporter PutP as a model, a complete Cys scanning mutagenesis of TM IX (positions 324 to 351) was performed here to further investigate the functional significance of the domain.