Within the whole cohort of analyzed embryos, six had no detectable mutation (detection threshold: 2%).
A rapid and reliable mutation detection assay has been developed.
HY and WC performed the plasmid construction, plant transformation and mutation detection.
We then performed mutation detection using denaturing high-performance liquid chromatography (DHPLC).
We observed allelic drop-out during LDLR genotyping and aimed at redesigning mutation detection.
CDH1/E-cadherin mutation detection rates vary between 12% and 83%.
The mutation detection rate for patients who fulfilled the Ghent criteria reached 71%.
To identify yeast mutants with a point mutation, detection of the specific mutant alleles is necessary.
A similar approach may be useful for making high-throughput in vivo models for mutation detection.
We have established a mobile phone-assisted microarray decoding platform for signal-enhanced mutation detection.
Currently available methods of EGFR mutation detection rely on direct sequencing.
