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Therefore, biochemical assays using enzyme levels rather than DNA mutation analysis are the tests of choice for initial Tay-Sachs screening in all population groups, especially in those not of Ashkenazi Jewish descent.
This suggests that when both sequencing and mutation analysis are 100% successful, 8% of patients (57 minus 49) would receive anti-EGFR therapy in vain when only exon 2 of KRAS would have been assayed.
Results for PTEN expression and PIK3CA mutation analysis are summarized in Table 2.
Primers and PCR conditions used in the mutation analysis are presented in table 1.
Detailed methods including bioinformatics mutation analysis are provided in the Supplementary Text.
Consequently, robust, reliable, and sensitive methods for mutation analysis are required to stratify patients eligible for anti-EGFR therapy.
Similar(47)
Mutation analysis was performed simultaneously.
Currently, EPCAM gene mutation analysis is not available in Japan.
Hence, mutation analysis is necessary prior to treatment.
Mutation analysis was performed to evaluate the capabilities of the test cases to detect faults.
Nevertheless, NOTCH3 gene mutation analysis is still costly and time-consuming [4, 14].
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