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Genome-wide cancer mutation analyses are revealing an extensive landscape of functional mutations within the noncoding genome, with profound effects on the expression of long noncoding RNAs (lncRNAs).
In clinical practice, mutation analyses are performed from a tissue sample available, preferably from a recently detected and resected metastasis.
In clinical practice, mutation analyses are performed from a tissue sample available, preferably from a recently detected biopsied or resected metastasis.
We deem that published in vivo TP53 mutation analyses are not as rigorous as studies in vitro, and we did not find any cell line showing a stable, single heterozygous mutation.
The results of the mutation analyses are listed in Table 2. Furthermore, we assessed whether TP53 missense mutants were transcriptionally active, on the basis of the IARC prediction models (http://p53.iarc.fr/MutationValidationCriteria.asp).asp
In Europe 50% to 70% of EGFR mutation analyses are performed on bronchial biopsy samples that usually contain a percentage of tumor cells that is lower than 50% [ 14].
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In addition, given that non- KRAS-mutated tumors include a distinct subset characterized by BRAF mutation, analyses were also performed to compare KRAS-mutated tumors with both BRAF-mutated tumors and the remaining subset of colorectal carcinomas, with observed neither somatic oncogene mutation.> -wrap-foot> Abbreviations: MMR mismatch repair, SD standard deviation.
Genomic DNAs for germ line mutation analyses were obtained from the buffy coat and resected tumor.
Mutation analyses were performed by PCR/sequencing.
Positive results from both mutation analyses were confirmed by sequencing.
To define the region that is responsible for the oncosuppression, mutation analyses were conducted.
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