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As shown in Figure 3B, AtBS14b ox plants developed shorter hypocotyls than wild type, so hypocotyl growth of AtBS14b mutants was further analyzed.

This increased thermotolerance of mutants was further enhanced by pre-exposure to a mild heat shock.

The panel of mutants was further utilized to define residues critical for neutralizing antibodies that recognize the V3 domain.

The complementary effect of Mg2+ on the swd p75fm and trpm7 b508 mutants was further evaluated for the proliferative ability of the exocrine pancreatic epithelia.

The different localisation of IQGAP1 observed in immunofluorescence studies of PANC-1 cells harbouring different Rac1 mutants was further analysed in coimmunoprecipitation experiments.

The pancreatic phenotype of the swd mutants was further characterized by examining the expression of the basic helix-loop-helix transcription factor ptf1a as a marker of exocrine pancreatic progenitors.

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IFABP-PX mutants were further optimized by introducing lysine residues in order to mimic the active site of native transaminases.

The circular mutants were further truncated one blade from the N- or C-terminus to generate new blade deletion mutants (X5123, X4512 and X3451).

Changes in the conformational behavior between the native HFE and its mutants were further analyzed by molecular dynamics simulations in the explicit solvent conditions.

These designed mutants were further fine-tuned through metabolic evolution resulting in the maximal biomass yield of 0.49 g-cdw/g-glucose, which matches well with predicted yield phenotype.

Mutants were further characterized after 4 rounds of backcrossing.

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