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The limit of detection of complex variants was further lowered by using haplotyping.
Structural basis for the difference in thermostability of wild type enzyme and its variants was further analyzed by computer modeling, and increasing in number of salt bridges and hydrophobic interactions around Lys209 was proposed as the main driving force for better compact protein structure of the enzyme.
The binding of urate to MftR variants was further investigated by measurement of intrinsic tryptophan fluorescence.
The identity of the variants was further confirmed by visual inspection of the tracks in integrative genomics viewer (IGV)[ 26].
The apparent deficiency in heme binding of these variants was further characterized by examining their visible electronic spectra, which more closely resembled that of free heme than that of hemoprotein.
We used SAMtools (version 0.1.18) [ 34] and the intersection to the initial GATK variants was further filtered for SNP quality (≥ 50); low and excessive read coverage (50 ≤ DP < 3000); presence of the reference allele; and homozygous non-reference calls in at least two of the 30 lines.
Similar(52)
The de novo variants were further assessed by their presence in exons (Tables 3 and 4).
Selected AcrB variants were further subjected thermofluor analyses (Cummings et al., 2006) to confirm that their thermostabilities were comparable to that of WT (Fig. S2B).
Fine-grained variants are further proposed by regarding two pieces as conflicting pieces if they perform conflicting accesses at runtime [13, 16, 41].
To analyze the homology to the reference proteins, C-terminal variants were further graded by the proportion of the amino acids in the reference sequences containing in the isoform, which presented as <25% (Very low), 25%-50% (Low), 50%-75 50%-75um) and >75% (High).
The top variants were further tested in an independent data set (751 cases and 751 controls).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com