Ai Feedback
Exact(9)
Among the truncated mutants, only the mutant AtGUS-t3 deleted a C-terminal hydrophilic coil peptide increased 3.8-fold catalytic efficiency with kcat/Km value of 8.31 mM−1·s−1.
In addition, we introduced a 31-mer peptide containing an auto-cleavage site to the N-terminal of the proteases and found the peptide could be cleaved efficiently by 3CLsc itself, but, among the four mutants, only the mutant Cys145 → Ser showed residual activity as detected by the auto-cleavage assay.
Of the tested tad-locus mutants, only the ΔrcpA mutant was unresponsive to IL-1β.
In most of the bub-1 fw8) bub-1 fw8s, only the two mutantsterionlyeam cells H0 were presentheFigure 4D).
In assays with Hsc70 truncation mutants, only the 1-540 species of Hsc70 bound to ICP27 (Figure 2B), indicating that the ATPase domain and the substrate binding domain of Hsc70 are required for interaction with ICP27 but not the CTD.
Concerning the three double mutants, only the combination of VPg and P3 substitutions induced symptoms that were qualitatively more severe than those produced by the single VPg L2013F mutant (Table 1) and almost as severe as those observed for TEV-At17.
Similar(51)
In the SOFL1-RNAi62 sonly-1 muthet, onlevels levels of specific cytokinins were affected.
A similar strategy was used to create the lsk1-K306R GFP tagged mutant, only the full length product was cloned into the EcoRI and SmaI sites of the pJK210-GFP vector.
In an F2 population for a recessive mutant, only the 25% mutant individuals will have a known genotype at the mutant locus, because the remaining wild-type plants can be either homozygous wild type or heterozygous.
ccdc40 mRNA injection rescued the severe reduction in epidermal flow in grinch mutants only on the injected side in 51/55 genotyped mutants.
For the double and triple mutants, only combinations of the individual mutations with a PFLU4744 mutation were positive for calcofluor and Congo red binding.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com