Sentence examples for mutants missing the from inspiring English sources

The structure of the LPS can be partially deduced by its size following electrophoresis because it is assembled sequentially and as a consequence, mutants missing the core or O-antigens will migrate faster.

As shown in Figure 4C, all mutants missing the C-terminus were quite stable in response to γ irradiation, while the wild-type and all N-terminal mutants were degraded in the extract prepared from the cells treated with γ irradiation.

Together, these findings may suggest that the ZF1 mutants missing the zinc-chelating residue are recognized as misfolded proteins and trapped by a molecular chaperone or degradation system (48, 49).

In this way, single mutants missing the first (N70, 'NQ1'), second (N119, 'NQ2'), third (N243, 'NQ3') N-glycosylation site or double and triple mutants missing two ('NQ12'NQ13Q13'NQ23Q23') or all three ('NQ123') sites were generated.

In particular the two mutants miss the CBM, due to the introduction of a stop codon between the catalytic module and the CBM at the position 249.

To determine the role of each residue of AChE586-599 in the process of amyloid formation, a library of alanine scanning mutants along with a structurally conserved substitution mutant (Tyr to Phe) and a truncation mutant (missing the last residue) were used.

Both halves of POSH pulled down full length c-Cbl, Cbl-b, and Cbl-b 2/3 (a mutant missing the N-terminal TKB domain).

One additional feature, the lack of two distal linker proteins, fits with the already known altered phycobilisome structure and may be the reason for the decreased phycocyanin content in mutant missing the bilin lyase.

The SUMO localisation likely corresponds to SUMO1 conjugated to substrate proteins because a non-conjugatable SUMO1 mutant missing the last two amino acids (SUMO1ΔGG) does not form an INB (Desterro et al. 2005; data not shown).

(C ) Western blot showing the recovery of endogenous OCRL in immunoprecipitates from cells expressing GFP-SNX9WT but not from cells expressing GFP-SNX9 mutant missing the SH3 domain (GFP-SNX9ΔSH3).

The remaining eight lysines were each individually mutated to alanine and tested for crosslinking with Fis-Q21C; a truncation mutant missing the two C-terminal lysines was also evaluated.