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In the case of strain Y51, CF significantly inhibited the growth of wild type cells, but did not inhibit the growth of the PCE-nondechlorinating LD and SD variants missing the pce gene cluster or pceA promoter sequence, respectively (Futagami et al. 2006b).
Additional variants were synthesized to incorporate the intron only, for td (two variants missing the first seven nucleotides [20] and containing shortened P6a regions [37]) and C.b. (12 circularly permuted variants possessing diverse P6a regions).
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Tromas and Elena (2010) also generated a TEV variant missing the NIb cistron, referred to here as TEV-Δ NIb.
Upon searching the NCBI database for murine B" family members via the BLAST algorithm, we retrieved the complete protein sequence of two murine PR72 isoforms: the human PR72 orthologue [GenBank: BAE21013] (referred to as mPR72/B α2) and a shorter variant missing the last 41 residues [GenBank: BAC28935] (referred to as mPR72/B α4).
This analysis shows that there were variants missing in the HuRef CG profile; undercalling of gains greater than 100 bp was particularly severe.
Annotation proceeds by collecting variants missing a respective annotation, annotating the list of variants, and storing the results in the database.
For each placement, we then apply the dynamic programming algorithm on the set of paired-end reads to infer the rare variants missed by the phased haplotypes.
Through cDNA sequencing and data mining, we found six natural variants missing cysteine residues involved in one or two disulphide bonds at the first reaction centre.
Such molecules, including truncated forms of neurexin I and neuroligin (i.e. mimicking autism variants, missing EF metal binding motifs), could serve regulatory roles by attenuating the function of full-length isoforms or behave as soluble signalling molecules [63], [70].
Splice variants missing Exons 3 and 5 are occasionally found, but Exons 4 and 8 are present in all variants sequenced thus far [ 22].
In addition, expression of Robo2 variants missing either Ig1 or Ig2 with slit-Gal4 did not result in any ectopic midline crossing.
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