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Exact(12)
All mutants listed in Figure 1B were modelled and the resulting structures were overlapped over the Wild Type.
The gene deletions in all ten mutants listed in Table 2 were confirmed by sequencing.
The 182 deletion mutants listed in [ 55] were excluded from further analysis.
Amplicons ABD12-22 and ABD2-9 were also analyzed by HRM using mutants listed in Table 2 and Additional file 1.
(A ) Diagram for screening miRNA deletion mutants (listed in Supplementary file 4 ) when fed mock or ced-3 RNAi to identify overt developmental phenotypes when ced-3 was depleted.
We note that, in general, the changes in activity for the mutants listed in Table 1 were due to a decrease in kcat values and that mutations that introduced steric clashes also demonstrated increases in KM.
Similar(48)
Only five genes showed evidence for significant coregulation of transcript and protein between wild-type and mutant (listed in Table S2).
Several of the original spo mutants proved to be hypomorphic alleles of essential genes (Nakase et al. 2001; Nakamura-Kubo et al. 2003) and so are not present in our deletion set; however we identified 13 known sporulation-defective mutants listed as present in the collection (Table 4).
Alternatively, the lipid composition of cell membranes might be altered in the mutants listed above, thereby changing the properties of the membrane and impacting on the activities of membrane-embedded transporters, sensors or signalling receptors [ 49].
Among the mutants listed above only FTN0757 was identified in our screen because of its exceptionally strong hypercytotoxic phenotype.
The 231 most rapidly proliferating mutants are listed in Table S9, and the 231 most slowly proliferating mutants are listed in Table S10.
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