(F) The RNF125 mutants used in Fig. 7.
The T-DNA insertion mutants used in this study are listed in Supplementary Table 2.
(C) Diagrams of the RNF125 stop mutants used in Fig. 2. (D) The C2HC ZnF mutant used in Fig. 3.
(E) Amino acid sequence of the C2HC ZnF for RNF125 and the mutants used in panels (E I).
We conclude that the RING and C2HC ZnF form a functional unit that is required for binding to the E2. Figure 1: RNF114, RNF114 and the mutants used in this study.
The mutants used in this study, Sphingobacterium sp. M4113/M4115, were originated from a wild-type strain GCG, which was isolated from the sewage of a styrene monomer-manufacturing factory.
Col-0 mutants used in this study include those deficient in lignin, cellulose, components of hemicellulose such as xylose and fucose, the pectic components arabinose and rhamnose, as well as mutants with enhanced arabinose and total pectin content.
The nomenclature of LinB mutants used in this study is shown in Table 1.
The A. thaliana ecotype Columbia (Col-0) and the mutants used in this study were obtained from the Arabidopsis Biological Resource Center (ABRC; http://www.arabidopsis.org): arc6 (AT5G42480, CS286) and arc12 (At1G69390, CS16472).
All mutants used in this study have been described previously.
The oamb mutants used in this study are the null alleles oamb286 and oamb96 [9].
