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αS mutants identified from inherited forms of Parkinson's disease were also studied for their impact on Ferriductase activity and iron reduction.
(C ) Norrin mutants identified from diseases show no binding to Fz4CRD.
This construct (P ANR -PsLAR) was transformed to wild-type Arabidopsis as well as ANR knock-out (anr) Arabidopsis mutants identified from a T-DNA knock-out database.
To verify the mutants identified from the deletion library, we independently confirmed all 33 mutant hits by PCR of the genomic-marker junctions created by the gene deletions.
Mutants identified from CAPS assay and SSCP were further confirmed by Sanger sequencing of amplicons (single copy genes) or performing PCR cloning with Zero-Blunt PCR cloning kit (Life Technologies, catalogue no. K 2700 20) prior to sequencing.
Mutants identified from the 384-colony array assays were individually verified by re-growing these mutants on the respective selective media or temperature as patches on regular Petri dish plates.
Similar(53)
We investigated the cardiac remodeling process of tr265/tr265, a zebrafish mutant identified from an ENU-mediated mutagenesis screen.
The D122A mutant, identified from structure-function studies of MC4R to be important for α-MSH binding [28], also responded robustly to THIQ (EC50 = 29.5 nM).
Among the eight genes upregulated in the zur mutant, identified from both microarray analysis and in silico screening, three encode putative TonB-dependent receptors (znuK, znuL and znuM).
By employing the best mutant identified from the screening (H359Y) the ee value of d- 2 a increased to greater than 99%% and the conversion to 78%% under the same reaction conditions, thus further demonstrating the faster production of d- 2 a with respect to wild-type PAL (other variants behaved similarly; see Table S4).
In other words, the mutants identified may prevent SpoIIE from being degraded and from being a proper phosphatase – I don't see the logic that SpoIIE must be transitioning from one state to another in terms of catalytic activity inside the forespore.
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